To examine the role of RUFY3 and its relationship with PAK1 in gastric cancer, we first investigated the protein levels of RUFY3 and PAK1 in gastric cancer cell lines (BGC-823, MKN45, AGS, MGC-803, SGC-7901 and MKN1) relative to the normal gastric epithelial cell line (GES-1) by western blot. The gene discussed is RUFY3; the disease is gastric cancer.