PAK1 and gastric cancer: To further seek the correlation between PAK1 and RUFY3 and explore the underlying mechanism of RUFY3-mediated gastric cancer cell migration, the increasing amounts of PAK1 expression plasmids were transfected, and the western blot results indicated that the protein levels of endogenous and exogenous RUFY3 were increased by the ectopic PAK1 expression level increasing (Figures 4a and b), suggesting that overexpression of PAK1 promotes RUFY3 expression.