The supporting evidence of LDH release, ROS production, MMP suppression, elevation of the level of cytochrome c, and activation of caspase-9 and -8 after suppression of the NF-κB signalling pathway demonstrate the promising anticancer activity of the CdCl2(C14H21N3O2) complex against the HT-29 colon cancer cell line via both intrinsic and extrinsic mitochondrial pathways. Here, CASP9 is linked to colonic neoplasm.