Additionally, screening at other targets of interest for AD treatment revealed that these hybrids can also inhibit in vitro butyrylcholinesterase (BChE), Aβ self-aggregation and BACE-1, the enzyme that catalyzes the first and rate-limiting step of the proteolytic cleavage of the amyloid precursor protein (APP) to Aβ, thereby expanding the in vitro multitarget profile of these compounds. This evidence concerns the gene BACE1 and Alzheimer disease.