As shown in Table 2, six imprinted genes showed different 5hmC patterns between normal and abnormal sperm (GDAP1L1, GNAS, KCNK9, LIN28B, RB1, RTL1), and five imprinted genes showed different 5hmC patterns between normal and globozoospermia sperm (KCNK9, LIN28B, RB1, SLC22A18, ZDBF2). The gene discussed is RB1; the disease is Globozoospermia.