Next, to determine whether the fusion of 3E8 and endostatin affects the binding ability to cells expressing TAG-72 antigen or endothelial cells, we analyzed the binding of 3E8, 3E8-mEndo, or mEndo to LS174T human colon carcinoma cells or HUVECs using flow cytometry. This evidence concerns the gene COL18A1 and colon carcinoma.