We found that garcinol treatment indeed caused the downregulation of constitutive NF-κB expression in a dose- and time-dependent manner (Figure 2A and 2B) and suppression of constitutive phospho-IκBα expression (Figure 2C) as well as NF-κB DNA binding activity (Figure 2D) in HNSCC cells. Here, NFKB1 is linked to head and neck squamous cell carcinoma.