Interestingly, we found that cisplatin can substantially increase nuclear p65 expression and phosphorylation level as well as NF-κB DNA binding activity in HNSCC cells, and both the constitutive and cisplatin-induced NF-κB activation could be suppressed upon garcinol treatment in HNSCC cells. This evidence concerns the gene NFKB1 and head and neck squamous cell carcinoma.