However, qPCR on p53-ChIP material from a neuroblastoma cell line with wild-type p53 (MYCN3) treated with nutlin-3 could not confirm direct binding of p53 to MIR182, MIR203, MIR222, and MIR432, whereas p53 binding to a positive control p53 target gene (CDKN1A) was clearly shown (figure 4B). Here, CDKN1A is linked to neuroblastoma.