Furthermore, as shown in Fig. 3, immunofluorescence imaging confirmed that hypoglycemia did not alter the localization of claudin-5, ZO-1, and occludin after 6, 12, or 24 h treatment, although occludin is displayed diffuse cytoplasmic localization in bEnd.3 (data not shown). This evidence concerns the gene TJP1 and Hypoglycemia.