To elucidate the underlying mechanism regulating BBB permeability during hypoglycemia and the potential role of exogenous VEGF in protecting brain endothelial cells against hypoglycemia, we established an in vitro BBB model using mouse brain microvascular endothelial cells (bEnd.3) which were cultured in normal glucose (5.5 mM glucose) or low glucose (0, 0.5, or 1 mM glucose) for varying times. This evidence concerns the gene VEGFA and Hypoglycemia.