In addition, in vitro experiments showed that F. alocis could induce apoptosis in gingival epithelial cells (GECs) and stimulate pro-inflammatory cytokines such as IL-1β, IL-6 and TNF-α secreted by GECs45, supporting the notion that F. alocis may be an important pathogen in the formation of subgingival biofilm and the onset of periodontal diseases. The gene discussed is IL1B; the disease is periodontal disorder.