Xiao et al (21) reported that 9L glioma cell spheres derived from mice were able to differentiate into cells positive for GFAP, neuron-specific enolase and Galc, which are representative markers of neuronal, astroglial and oligodendroglial cells; however, in humans, such markers differentiated from SLCs were GFAP, β-tubulin III and Galc (22). This evidence concerns the gene ENO2 and glioma.