In summary, we observed the highly expressed TACC3 in ESCC and knockdown TACC3 contributed decreases in cell proliferation, colony formation ability and EMT of ESCC cells, and demonstrated that ESCC patients expressing high levels of TACC3 exhibit a substantially lower 5-year overall survival rate than TACC3-low expression patients. The gene discussed is TACC3; the disease is esophageal squamous cell carcinoma.