TACC3 and esophageal squamous cell carcinoma: To investigate the TACC3 expression in ESCC, qRT-PCR and western blotting were performed in both an immortalized normal human esophageal epithelial cell line, NE3, and a panel of ESCC cell lines including Eca-109, EC18, HKESC1, KYSE30, KYSE140, KYSE150, KYSE410, and KYSE510, respectively.