However, behavioral testing of wild-type and constitutive Htr1a knockout littermates deriving from a separate breeding confirmed the anxiety phenotype of the knockout mice (Ramboz et al., 1998; Bonasera and Tecott, 2000; Ase et al., 2002; Toth, 2003; Piszczek et al., 2013) under the breeding and testing conditions used in this study (Supplementary Figure S2). This evidence concerns the gene HTR1A and Anxiety.