Unsupervised hierarchical clustering of titin peptides from control and DCM cardiomyocytes infected with U7snRNA-ScrAONs-IRES-GFP highlighted the presence of a cluster, which was significantly enriched in exon 326 peptides and down-regulated in the diseased cells, as expected (Fig2C). This evidence concerns the gene TTN and familial dilated cardiomyopathy.