CDH1 and cancer: To determine whether altering actin cytoskeleton dynamics could directly affect EMT we treated six metastatic human cancer cell lines (prostate Du145, breast MDA-MB-231 and MBA-MB-435, glioma U251 and U87 and fibrosarcoma HT-1080) with the actin depolymerising agent Cyt D. Cells exposed to Cyt D (100 μM) for 12 h showed a reduced size and stained positively for E-cadherin (Fig. 1A).