HMGB1 and colorectal cancer: From a more clinical perspective, Frey et al. reported that chemotherapeutic agents such as 5-FU, oxaliplatin, and irinotecan (which are used for adjuvant chemotherapy for colorectal cancer), alone or in combination with X-irradiation, induce the release of HMGB1 from a colon adenocarcinoma cell line, SW480, in vitro [27].