A CpG-rich region in the promoter and exon 1 of PRSS8 is heavily methylated when this gene is repressed in MDA-MB-435 breast cancer cells (62), and demethylation by 5-aza-2′-deoxycytidine combined with histone deacetylase inhibitor treatment reactivates expression of PRSS8. In our studies, Double Mutant MBD2 protein exerts diminished repression of PRSS8 when compared to an equivalent amount of wild-type MBD2 protein, (Figure 6c) thus confirming the importance of these two amino acid residues in MBD2-NuRD function. The gene discussed is PRSS8; the disease is breast cancer.