Here we use qPCR to measure the presence of six pathogens, previously reported in association with CRC, in paired adenocarcinoma and adjacent normal mucosal samples; these include Fusobacterium spp., Streptococcus gallolyticus, Enterococcus faecalis, ETBF, EPEC and afaC- or pks-positive E. coli. Here, ARAF is linked to colorectal carcinoma.