Among the remaining patients in the GSE3494 cohort (n = 217, validation cohort) we validated the capacity of the p53 gene signature to predict TP53 status, and found that the p53 signature remained significantly associated with tumor p53 status using both t-tests and receiver operator characteristic (ROC) curve analysis (Figures 5B–5C, t-test, *p < 0.0001, AUC: 0.74, *p < 0.0001). This evidence concerns the gene TP53 and neoplasm.