To determine if the G9a-Snail complex in the E-cadherin promoter is essential for E-cadherin repression in HNSCC, we examined the effects of an inhibitor of G9a, BIX01294, on the H3K9me2 methyltransferase activity in the TGF-β-induced EMT model. This evidence concerns the gene CDH1 and head and neck squamous cell carcinoma.