To elucidate the effects of FBXW7 on the EMT of CCA cells, we retrovirally established stable silencing of FBXW7 in HuCCT1 and RBE cells (designated as HuCCT1-shFBXW7.1, HuCCT1-shFBXW7.2, RBE-shFBXW7.1 and RBE-shFBXW7.2), and overexpression of FBXW7 in QBC939 and FRH0201 cells (designated as QBC939-FBXW7 and FRH0201-FBXW7). The gene discussed is FBXW7; the disease is cholangiocarcinoma.