To test the function of FBXW7 on EMT related cell behaviors in CCA cells, wound healing assay was firstly carried out, which showed markedly stimulated migration of the HuCCT1-shFBXW7 cells and RBE-shFBXW7 cells compared with their respective control cells (Fig. 3A; Supplementary Fig. S2A). Here, FBXW7 is linked to cholangiocarcinoma.