IHC analysis of the tumor specimens exhibited a significant loss of E-cadherin expression in metastatic IHCC and PHCC (Fig. 2A) and a positive correlation between the expression of FBXW7 and E-cadherin in IHCC and PHCC (Fig. 2B), indicating FBXW7 may be involved in the EMT of CCA. This evidence concerns the gene CDH1 and cholangiocarcinoma.