We measured mRNA levels of genes encoding for several markers of inflammation related to obesity, including the chemokine MCP1 (Ccl2), proinflammatory cytokine IL6 (Il6) and TNFα (Tnf), and macrophage-specific marker F4/80 (Emr1) in adipose depots (Figure 2D & E). This evidence concerns the gene TNF and obesity due to melanocortin 4 receptor deficiency.