Among the patients carrying the NKX2-5 p.R25C missense, patient F with ASD has a de novo BMPR2 nonsense mutation, patient G with TOF has an in-frame deletion in SOS1 and a missense in NKX2-6 both inherited from the second parent, and patient I with PDA has a missense in GATA4 inherited from the second parent (Sup. This evidence concerns the gene SOS1 and atrial septal defect.