Till date, more than 1000 different mutations have been identified in patients with OI, and these mutations are distributed in over ten different genes, such as COL1A1, COL1A2, CRTAP, LEPRE1, and FKBP10. Since the classical genetic analysis methods, like capillary sequencing, are time-consuming and expensive, it necessitates the development of an efficient customized sequencing platform in order to perform genetic sequence analysis more rapidly, and accurately, and simultaneously for multiple genes. The gene discussed is COL1A1; the disease is osteogenesis imperfecta.