In vitro, DM-1 also induced apoptosis via the extrinsic pathway by TNF-R1 and cleaved caspase-8 increase and by the intrinsic pathway via Bcl-2/Bax ratio decrease, cytochrome-c release and the electric mitochondrial membrane potential decrease in melanoma cells [9,10]. The gene discussed is TNFRSF1A; the disease is melanoma.