Of note, several microRNAs encoded in the DLK1-MEG3 cluster (Figure 1) within RTL1 (for example, miR127, miR136, miR431 and miR433) and between RTL1 and DIO3 (miR376a, miR487b, miR382, miR380-5p and miR412) have also been described to be significantly reduced or silenced by DNA hypermethylation in bladder tumor tissues and the cell lines RT4, RT112 and T24 [19, 29] suggesting that silencing may extend across a large part or the entire imprinted gene cluster at 14q32. This evidence concerns the gene DIO3 and urinary bladder neoplasm.