NSCLC cell lines expressed SP17/AKAP4/PTTG1 transcripts and proteins, while the non-tumorigenic cell line showed only weak SP17 and PTTG1 expression in immunofluorescence but was negative by immunocytochemistry, possibly due to the higher sensitivity of immunofluorescence. The gene discussed is PTTG1; the disease is non-small cell lung carcinoma.