We used our lentiviral system to silence UbcH10 gene in the resistant cell lines and the parent cell line, and examined their proliferation, cell cycle distribution, sensitivities to chemotherapeutics, and apoptosis, to evaluate the effects of gene inference on tumor cells, and to investigate the possibility of using the UbcH10 gene as a therapeutic target in breast cancer. This evidence concerns the gene UBE2C and breast cancer.