Unsupervised clustering with this signature using the Boyault et al. dataset [12] revealed two major classes, one that exhibited higher expression of Wnt transcriptional targets and lower expression of RXR-α transcriptional targets (Wnt high, RXR-α low), and the opposing group (Wnt low, RXR-α high) (Fig. 4), similar to the classification we observed with the signature in mouse HCC (S2 Fig). This evidence concerns the gene RXRA and hepatocellular carcinoma.