Furthermore, NOTCH3 DNA copy number is positively correlated with NOTCH3 protein expression based on parallel immunohistochemistry and FISH studies in 111 high-grade tumors.23 However, another study NOTCH1 gene polymorphism is associated with the T type leukemia, but the genotypes of NOTCH2, NOTCH3 and NOTH4 genes are not associated with human cancers.24 Our study showed that the 684G>A polymorphism affects the tumor NOTCH3 expression level and is closely associated with a higher tumor grade, poorer tumor differentiation and KPS scores in these gliomas patients. Here, NOTCH1 is linked to central nervous system cancer.