To analyze more deeply the effect of C/EBPβ depletion on the ability of GBM cells to generate new spheres, actively growing 7 day-old GL261-derived primary GNSF cultures were dissociated, and equal numbers of viable cells were replated in fresh neurosphere medium to generate new GNSF (secondary glioblastoma-derived neurospheres). Here, CEBPB is linked to glioblastoma.