To test whether autophagy acts as a cytoprotective mechanism in our system, we inhibited autophagy in CML cells using LY294002, chloroquine (CQ) and quinacrine (QN) [34, 35], and analyzed the effects on the level of LC3-II and asparaginase-induced cell death. The gene discussed is ASPG; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.