Finally, we looked at RIG-I activation by fluorescence microscopy and we observed an enhanced punctuated staining of RIG-I upon Sp1 induction (-DOX: 91±7% vs +DOX: 42,2±11,7%) (Fig. 3B), suggesting the formation of RIG-I aggregates as it was described for MAVS activation in a context of viral infection [40]. Here, SP1 is linked to viral infectious disease.