To assess the efficiency of these newly engineered Ads to suppress c-Met, multiple human glioma cell lines (U251N, U343, and U87MG) and human normal fibroblast cell line (HDF) were transduced with dl/LacZ, dl/shMet4, dl/shMet5, or dl/shMet4+5. The gene discussed is MET; the disease is glioma.