Expression of miR-CFL1 through the infection of Lenti-CMV-ChR2-eYFP-miR-CFL1_1 or-CFL1_2 did not affect the mRNA levels of these genes (ADF; miR-CFL1_1, p = 0.11; miR-CFL1_2, p = 0.50: Twinfilin1; miR-CFL1_1, p = 0.66; miR-CFL1_2, p = 0.99: Twinfilin2; miR-CFL1_1, p = 0.86; miR-CFL1_2, p = 0.69: versus miR-Neg, Dunnett’s multiple comparison test), although CFL1 expression significantly decreased (miR-CFL1_1, p = 2.6 × 10-7; miR-CFL1_2, p = 5.2 × 10-7 versus miR-Neg) (Fig. 2J). This evidence concerns the gene DSTN and infection.