To examine the possible tyrosine kinase overexpression in the Tgfbr1/Pten 2cKO mouse model of HNSCC, we used a high-throughput antibody array with 39 RTKs to test the RTK expression of Tgfbr1/Pten 2cKO mouse tongue SCC in comparison with those of Tgfbr1/Pten 2cKO mouse tongue and Tgfbr1flox/flox/Ptenflox/flox tongue. The gene discussed is PTEN; the disease is head and neck squamous cell carcinoma.