We would measure serum levels of proinflammatory cytokines, namely, IL-1α, IL-1β, IL-6, and TNF-α in TRAMP mice, by using a highly sensitive commercial multiplex suspension array technology kit (FlowCytomix) and flow cytometry, in an attempt to define their potential effect in the association between HFD and TRAMP PCa development and progression. This evidence concerns the gene TNF and posterior cortical atrophy.