NFATC2 and neoplasm: Immunohistochemical analysis of NFAT1 in 4T1 tumors revealed the activation of the Cn/NFAT pathway, as NFAT1 was detected in the nuclei (black stars) of control tumor cells, whereas it was exclusively found in the cytoplasm of shCnB1-silenced tumors and not detected in shNFAT1-silenced tumors (Figure 3a).