PPP3R1 and neoplasm: To next determine whether the Cn/NFAT pathway is essential to tumorigenesis, the control and CnB, NFAT1 or NFAT2-silenced 4T1 cells were injected into the mammary gland of syngenic Balb/c mice and tumor growth was monitored every 3 days over a period of 3–6 weeks, time after which mice were killed.