MAB21L2 and coloboma: Both increased cell death (through apoptosis and/or necroptosis) and defects in cellular proliferation have been linked to optic fissure closure defects in other genetic models of coloboma [48–59]; since the TUNEL assay may not discriminate between apoptotic and other cell death mechanisms [60], further studies are needed to evaluate the possible involvement of necroptosis in mab21l2 deficient phenotypes.