RASAL1 and renal fibrosis: To further substantiate the link of aberrant Rasal1 promoter methylation, progressive renal fibrosis and the reno-protective efficacy of de-methylating compounds 5′-Azacytidine and Hydralazine, we next challenged transgenic mice harboring transgenes for doxycycline-inducible RASAL1 over-expression (rtTAhCMV;hRASAL1-pTreTight) with UUO (Supplementary Fig. 4A, B).