EGFR and neoplasm: Four methods of estimating EGFR concentrations in normal muscle (no EGFR), U251 tumor (moderate EGFR) and A431 tumor (high EGFR) tissues were compared: 1) targeted NP concentration after 10 rinses, BP estimation via a ratio of targeted to untargeted NP concentration (BPRatio) after 10 rinses, BP estimation via dual-probe model analysis without nonspecific binding (BPDPM), and BP estimation via dual-probe model analysis WITH nonspecific binding (BPDPM-NS).