To determine whether the increase of SNORD76 following the HOTAIR siRNA-expressing lentivirus treatment was coincidental, we used quantitative PCR to evaluate the expression of HOTAIR, SNORD76, and GAS5 in 5 glioma cell lines (U87, U87-EGFRvIII, LN229, U251 and SNB19). The gene discussed is GAS5; the disease is central nervous system cancer.