In order to determine the isoform specific role of GSK3 in the regulation of prostate cancer cell growth and proliferation, we generated PC3, DU145 and LNCaP stable cell lines expressing ShRNAs for control (non-target), GSK3α and GSK3β (ShControl, ShGSK3α and ShGSK3β, respectively). This evidence concerns the gene GSK3A and Familial prostate cancer.