It was shown that active immunization with the octa-valent mixture of IsaA-His6, LytM-His6, Nuc-His6, His6-pro-Atl, and PSMα1-4, although resulting in high anti-staphylococcal IgG levels, did not protect mice against mild S. aureus isolate P bacteremia, severe S. aureus USA300 bacteremia, or S. aureus isolate P skin infection. The gene discussed is NUCB1; the disease is bacterial infectious disease with sepsis.