Recently, a multi-assay algorithm (MAA) has been suggested that includes 1) BED-capture EIA at a higher cutoff as the first step, followed by 2) Bio-Rad Avidity EIA, again at a higher cutoff 3) then CD4 measurement, and 4) finally VL measurement to classify recent HIV infection[52,59,60]. The gene discussed is MLC1; the disease is HIV infectious disease.