Given that, we first constructed pmiR-REPORT luciferase vectors containing wild-type or mutant 3’-UTR of PDRG1 inserted between the hRluc and the hLuc gene, and then carried out Dual-Luciferase reporter assay by cotransfecting above vectors with miR-214 mimics or miR-NC into bladder cancer cells. The gene discussed is PDRG1; the disease is urinary bladder carcinoma.