To test whether ectopic expression of SATB1 could induce EMT in non-metastatic tumor cells, the human bladder cancer cell line (BIU-87) with very low SATB1 expression was transfected with pcDNA3.1-SATB1 expression plasmids to establish a stable SATB1-overexpressing cell line as described in Material and Methods. The gene discussed is SATB1; the disease is urinary bladder carcinoma.