Subsequent RT-PCR or Western blot analysis showed that multiple types of human cancer cell lines, including cholangiocarcinoma [46], testicular seminomas [47], human breast cancer [48–50], cervical cancer [50], prostate cancer [50], and colorectal Cancer [53, 54], expressed higher NEK2A in the level of mRNA or protein than normal human fibroblast cells. This evidence concerns the gene NEK2 and cancer.