NEK2 and cancer: Consistently, analysis by Western blot, real-time PCR, DNA microarray, and immunohistochemistry indicated that increased NEK2A is found in various cancer tissues, such as human testicular seminomas [47], human breast carcinoma [49, 51, 52], colorectal cancer [53, 54], malignant peripheral nerve sheath tumors [55], nonsmall cell lung cancer [56], renal cell carcinoma [57], and pancreatic ductal adenocarcinoma [58].