This result was corroborated by western blots for all seven histone marks in additional Ewing sarcoma cell lines (TC252, SK-N-MC, and ST-ET-7.2), where we observed a global reduction in H3K4me3 and H3K27ac levels upon knockdown of EWS-FLI1 (Figure 4E). This evidence concerns the gene FLI1 and Ewing sarcoma.