A recent study suggested that neferine can induce autophagy, ROS hyper-generation, PI3K/Akt/mTOR inhibition and cytotoxicity in lung cancer cells [17]; however, the role of PI3K/AKT/mTOR inhibition on autophagy induction, as well as on the neferine-mediated cytotoxicity remains unclear. Here, MTOR is linked to lung cancer.