The expression of Nischarin protein in cancer tissues from various grades of invasive ductal carcinoma were evaluated, and no significant differences were detected between each grade (grade I, 5.44±3.57; grade II, 6.42±3.85; grade III, 5.10±1.18 ng/ml; P=0.765; Fig. 3). The gene discussed is NISCH; the disease is cancer.