The results indicated that: i) Nischarin expression was significantly lower in breast cancer tissues compared with that of normal tissues; ii) Nischarin expression levels were significantly lower in patients with lymph node metastasis compared with those of patients without lymph node metastasis; and iii) there was no significant difference in Nischarin expression levels between patients with grades I, II or III breast cancer. Here, NISCH is linked to breast carcinoma.